Research Opportunities

Invenio - Handheld Label-free Histology Device

SBIR Award Title

SBIR Phase II: Dual-Wavelength Picosecond Fiber Laser Source for Label-Free Microscopy

SBIR Award Abstract

This Small Business Innovation Research (SBIR) Phase II project aims to develop a novel research microscope based on coherent Raman scattering (CRS). In contrast to other techniques, CRS is a label-free method that requires excitation with two synchronized laser pulse trains (ultra-short pulse duration) with precisely tunable wavelengths (<1nm). The key innovation of the Phase I proposal was the realization that the difference frequency of the two major gain media used in the telecommunication industry, Erbium and Ytterbi... more

Research Opportunity

Handheld Label-free Histology Device


2310 Walsh Avenue
Santa Clara


Christian Freudiger

Principal Investigator

Christian Freudiger


In 2017, Invenio Imaging will be launching an ex vivo embodiment of our high-speed label-free chemical imaging technology, Stimulated Raman scattering Histology (SRH). Raman spectroscopy allows non-destructive characterization of the chemical composition of a sample based on the intrinsic spectroscopic properties of the molecular bonds. Raman imaging is possible by raster scanning the excitation laser through the sample and generating a point-by-point three-dimensional chemical map. While the chemical specificity of traditional Raman spectroscopy is excellent, the signal is extremely weak. This limits spectral analysis to high-concentration species and the acquisition rates in imaging applications to minutes and hours. Stimulated Raman scattering (SRS) provides amplification of the weak spontaneous Raman signal by >10,000x enabling high-speed, label-free chemical imaging at speeds up to video-rate (30 frames/s).
We have built prototype handheld SRS systems. The goal of this project would be to fully develop one or more handheld embodiments suitable for clinical use, principally for intraoperative tumor margin delineation.

Desired Knowledge

The handheld device comprises a sophisticated objective lens and relay optics, and a resonant scanning mechanism. Experience in optics, mechanics, electrical systems and basic computer programming is recommended. In addition, knowledge of, and/or interest in, the medical use cases would be beneficial. Assuming technical success, the research follow could also participate in preparation of documentation for submission to the FDA, in presentations to prospective corporate partners and prospective investors..
Invenio is a small company. To be successful, the candidate should be independent and willing to take on unfamiliar tasks.